Effect of enzyme-assisted extraction on yield, composition, and antimicrobial activity of essential oils from Rosmarinus officinalis L. grown in Lam Dong Province, Viet Nam

In the present study, viscozyme L pre-treatment for aerial parts of Rosmarinus officinalis L. (rosemary), grown in Lam Dong province, was performed to evaluate effective extraction essential oils by hydro-distillation. Enzymatic with 1 g enzyme mixed 5 mL sodium chloride 15% at 50oC hour, followed hydro-distillation, seen slightly increase yield rosemary from 0.96% 1.08%. GC/MS analysis showed presence 32 compounds oils, which major components were α-pinene (29.71 - 32.17%) and cineol (17.55 18.74%) both control enzymatically treated samples. The results also revealed that obtained samples exhibited moderate antimicrobial activity against Escherichia coli, Staphylococcus aureus, Candida albicans all tested concentrations.